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HETEROKARYON INCOMPATIBILITY GENES IN NEUROSPORA CRASSA DETECTED USING DUPLICATION-PRODUCING CHROMOSOME REARRANGEMENTS
Orest M. Mylyk 1
1 Department of Biological Sciences, Stanford University, Stanford,
California 94305
Evidence is presented for five or six previously undetected heterokaryon
incompatibility (het) loci, bringing to about ten the number of such
genes known in Neurospora crassa. The genes were detected using chromosome
duplications (partial diploids), on the basis of properties previously known
for het genes in duplications. Duplications homozygous for
het genes are usually normal in growth and morphology, whereas those
heterozygous are strikingly different. The heterozygotes are inhibited
in their initial growth, produce brown pigment on appropriate medium, and
later "escape" from their inhibition, as a result of somatic events, to produce
wild-type growth.—Five normal-sequence strains were crossed to 14 duplication-producing
chromosome rearrangements, and the duplication progeny were examined for properties
characteristic of duplications heterozygous for known het genes.
Each cross produced duplications for a specific region of the genome, depending
on the rearrangement. Normal-sequence strains were wild types from nature,
chosen from diverse geographic locations to serve as sources of genetic variation.—The
duplication method was very effective. Most of the longer duplications uncovered
het genes. The genes are: het-5 (on linkage group IR, in the
region covered by duplications produced using rearrangement T(IR
VIR)NM103
), het-6 (on IIL, covered by T(IILVI)P2869 and
T(IILIIIR)AR18 duplications), het-7 (tentatively assigned
to IIIR, T(IIIRVIL)D305), het-8 (VIL, T(VILIR)T39M777
), het-9 (VIR, T(VIRIVR)AR209), and het-10
(VIIR, T(VIIRIL)5936).
Revised on January 3, 1975
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