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RECESSIVE LETHAL AMBER SUPPRESSORS IN YEAST
Marjorie C. Brandriss 1, Larry Soll 1, and David Botstein 1
1 Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Recessive lethal amber suppressor mutations have been isolated in a diploid strain of Saccharomyces cerevisiae. Diploids carrying these suppressors upon sporulation yield asci with only two live spores, both lacking the suppressor. At least two classes of recessive lethal suppressors exist. Aneuploid strains carrying one wild type and one suppressor locus have been isolated and used in mapping studies; one suppressor maps on chromosome III, the other does not.
Submitted on June 10, 1974Revised on December 4, 1974