- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by O'Donnell, J.
- Articles by Sofer, W.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by O'Donnell, J.
- Articles by Sofer, W.
CHEMICAL SELECTION OF MUTANTS THAT AFFECT ALCOHOL DEHYDROGENASE IN DROSOPHILA II. USE OF 1–PENTYNE–3–Ol
Janis O'Donnell 1, Larry Gerace 1, Flora Leister 1, and William Sofer 1
1 Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218
We describe a procedure for the selection of alcohol dehydrogenase negative mutants in Drosophila. The method consists of exposing eggs and larvae to low concentrations of 1-pentyne-3-ol dissolved in the culture medium. Only those flies with greatly reduced levels of alcohol dehydrogenase activity survive. In addition, genotypically negative flies die if their mothers are alcohol dehydrogenase positive. Using this procedure and formaldehyde to generate mutants, we were able to detect seven alcohol dehydrogenase negative mutants out of 350,000 individuals subjected to selection. At least five of the mutants contain small deletions that include the alcohol dehydrogenase locus.
Submitted on April 18, 1974Revised on July 27, 1974
This article has been cited by other articles:
 |
|
 |
|
  S K Logan and P C Wensink Ovarian follicle cell enhancers from the Drosophila yolk protein genes: different segments of one enhancer have different cell-type specificities that interact to give normal expression. Genes & Dev., April 1, 1990; 4(4): 613 - 623. [Abstract] [PDF]
|
|
|
|
|
|
C Wilson, R K Pearson, H J Bellen, C J O'Kane, U Grossniklaus, and W J Gehring P-element-mediated enhancer detection: an efficient method for isolating and characterizing developmentally regulated genes in Drosophila. Genes & Dev., September 1, 1989; 3(9): 1301 - 1313. [Abstract] [PDF]
|
|
|
|
 |
|
 D. H. JOHNSON and T. B. FRIEDMAN Purine Resistant Mutants of Drosophila Are Adenine Phosphoribosyltransferase Deficient Science, May 29, 1981; 212(4498): 1035 - 1036. [Abstract] [PDF]
|
|
|
|
 |
|
 W. Lukowitz, T. C. Nickle, D. W. Meinke, R. L. Last, P. L. Conklin, and C. R. Somerville Arabidopsis cyt1 mutants are deficient in a mannose-1-phosphate guanylyltransferase and point to a requirement of N-linked glycosylation for cellulose biosynthesis PNAS, February 27, 2001; 98(5): 2262 - 2267. [Abstract] [Full Text] [PDF]
|
|