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GENETIC ANALYSIS OF MUTATIONS AFFECTING GROWTH OF SACCHAROMYCES CEREVISIAE AT LOW TEMPERATURE
Arjun Singh 1 and T. R. Manney 2
1 Department of Microbiology, Case Western Reserve University, Cleveland, Ohio 44106
2 Donner Laboratory, University of California, Berkeley, California 94720
A large number of genes control growth of the yeast Saccharomyces cerevisiae at low temperatures (< 10°). Approximately 47 percent of the mutants selected for inability to grow at 4–5°C show increased sensitivity to cycloheximide. In 3 of 4 cases tested, supersensitivity to cycloheximide and inability to grow at the low temperature segregate together and thus appear to be effects of the same mutation. Since many cold-sensitive mutants of bacteria have been found to have altered ribosomes and since cycloheximide resistance in yeast can be caused by ribosomal changes, this suggests that the mutants having low-temperature-sensitive growth may be defective in ribosome-assembly processes at the low temperatures. Two of the lts loci, lts1 and lts3 have been located on chromosome VII and another two, lts4 and lts10 on chromosome IV. A mutation, cyh10, conferring cycloheximide resistance, but not cold sensitivity, has been located close to the centromere on chromosome II.
Submitted on February 5, 1974
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