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THE DISTRIBUTION OF CROSSOVERS ALONG UNREPLICATED LAMBDA BACTERIOPHAGE CHROMOSOMES
Franklin W. Stahl 1, Kenneth D. McMilin 1, Mary M. Stahl 1, Jean M. Crasemann 1, and Stephen Lam 1
1 Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403
The distribution of crossovers along unreplicated chromosomes of bacteriophage lambda has been examined by determining the density distributions and genotypes of particles in the progenies of crosses of density-labeled by ordinary parents in the presence of genetic blocks to replication. The Red and Rec systems combined produce crossovers primarily near the ends (especially the right end) of the chromosome. Removal of the generalized lambda recombination functions by red and gam mutations results in loss of these terminal crossovers; coupled with this loss is a disappearance of the differential dependence of recombination frequencies in terminal and central intervals on DNA synthesis. Removal of the bacterial system by a recA mutation results in severe depression of crossing over among unreplicated phage, with the few recombinants produced by the lambda system occurring near the right end.
Submitted on December 28, 1973
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