RECOMBINANTS BETWEEN CLOCK MUTANTS OF CHLAMYDOMONAS REINHARDI

Victor G. Bruce ¹

¹ Department of Biology, Princeton University, Princeton, N. J. 08540

Mutants affecting the period length of the biological clock in Chlamydomonas reinhardi have been isolated and a start has been made on analyzing the genetics of this system. In four mutants, the long period characteristic seems to be controlled by single genes at separate loci. Crosses between single mutants, as well as crosses involving three or four mutant genes, yielded progeny with periods characteristic of the parents as well as recombinant types, including normal period (wild type) and extra-long periods (double, triple and quadruple mutants). It was found that the period lengthening effect is additive; that is, the period of double mutants is lengthened by the sum of the period lengthening of the single mutants.

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