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SOME IMPROVED METHODS IN P22 TRANSDUCTION
Bert Ely 1, Roger M. Weppelman 1, Holman C. Massey Jr. 1, and Philip E. Hartman 1
1 Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218 and Department of Biochemistry, University of California, Berkeley, California 94720
Recent refinements simplify methods for P22 transduction in Salmonella and allow improved recovery of phage-free transductional clones. The methods include use of: integration- and lysis-defective phage mutants, heat-killed bacteria to eliminate free phage, direct plating of phage and bacteria, replica-plating for detection of phage content of individual clones, improved broth for phage growth, and procurement of high titer phage from P22 lysogens.
Submitted on November 26, 1973
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