- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by White, K.
- Articles by Sueoka, N.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by White, K.
- Articles by Sueoka, N.
TEMPERATURE-SENSITIVE DNA SYNTHESIS MUTANTS OF BACILLUS SUBTILIS—APPENDIX: THEORY OF DENSITY TRANSFER FOR SYMMETRIC CHROMOSOME REPLICATION
Kalpana White 1 and Noboru Sueoka 1
1 Departments of Biochemical Sciences and Biology, Princeton University Princeton, New Jersey 08540
A simple method for characterizing temperature-sensitive DNA synthesis mutants is described. The method uses density transfer and transformation techniques and is based on expected theoretical behavior of the chromosome population. A direct proof of inhibition of initiation of DNA replication is provided. The mutant dna-1, showing quick inhibition of initiation, is further characterized and mapped. An independent method for mapping genetic markers close to the origin, based on their transfer behavior after inhibition of initiation, is presented.
Submitted on January 31, 1972Revised on November 6, 1972