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NUCLEIC ACID HYBRIDIZATION STUDIES WITHIN THE GENUS CUCURBITA
Robert B. Goldberg 1, William P. Bemis 1, and Albert Siegel 1
1 Committee on Genetics and Departments of Horticulture and Agricultural Biochemistry, The University of Arizona, Tucson, Arizona 85721
The DNAs of Cucurbita species were examined by several methods. All Cucurbita DNAs have a similar CsCl isopycnic banding pattern consisting of a major band at 1.695 g/cc and a well separated satellite band at 1.707 g/cc. Compared to other plant and animal genera, Cucurbita species have a large genomic proportion of rDNA; this value ranging from 1.4% to 3.1%. The genomic proportion of rDNA was found not to be useful as a characteristic indicating degree of relatedness of the various Cucurbita species. However, Cucurbita DNAs can be distinguished by the extent to which their repetitive sequences cross-hybridize to each other and an assessment of species relationships can be made on this basis.
Submitted on March 22, 1972Revised on May 9, 1972
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