CYTOCHEMICAL STUDIES ON THE PROBLEM OF MACRONUCLEAR SUBNUCLEI IN TETRAHYMENA

John Woodard 1, Edna Kaneshiro 1, and Martin A. Gorovsky 1

1 Whitman Laboratory, University of Chicago, Chicago, Illinois 60637

DNA amounts in macronuclei and micronuclei of Tetrahymena pyriformis were measured by Feulgen microspectrophotometry. Assuming that the unreplicated micronucleus is diploid, the unreplicated macronucleus was found to contain approximately 45 times the haploid DNA amount. The relationship of these findings to the 45 independently assorting genetic subunits characterized by Allen and Nanney and their collaborators is discussed. The pattern of DNA synthesis in macro- and micronuclei during the cell cycle is also described.

Submitted on September 7, 1971
Revised on November 4, 1971




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