- THIS ARTICLE
- Full Text
- Full Text (PDF)
- Supporting Information
-
All Versions of this Article:
genetics.109.107268v1
184/2/587 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Email this article to a friend
- Related articles in Genetics
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Eng, K. H.
- Articles by Gasch, A. P.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Eng, K. H.
- Articles by Gasch, A. P.
Originally published as Genetics Published Articles Ahead of Print on December 4, 2009.
Genetics, Vol. 184, 587-593, February 2010, Copyright © 2010
doi:10.1534/genetics.109.107268
Transient Genotype-by-Environment Interactions Following Environmental Shock Provide a Source of Expression Variation for Essential Genes
Kevin H. Eng*,1,
Daniel J. Kvitek
,1,2,
Sündüz Kele
*,
and
Audrey P. Gasch,
,3
* Department of Statistics, Laboratory of Genetics, Department of Biostatistics and Medical Informatics and Genome Center of Wisconsin, University of Wisconsin, Madison, Wisconsin 53706
3 Corresponding author: Laboratory of Genetics, 425-g Henry Mall, University of Wisconsin, Madison, WI 53706.
E-mail: agasch{at}wisc.edu
Understanding complex genotype-by-environment interactions (GEIs) is crucial for understanding phenotypic variation. An important factor often overlooked in GEI studies is time. We measured the contribution of GEIs to expression variation in four nonlaboratory Saccharomyces cerevisiae strains responding dynamically to a 25°–37° heat shock. GEI was a major force explaining expression variation, affecting 55% of the genes analyzed. Importantly, almost half of these expression patterns showed GEI influence only during the transition between environments, but not in acclimated cells. This class reveals a genotype-by-environment-by-time interaction that affected expression of a large fraction of yeast genes. Strikingly, although transcripts subject to persistent GEI effects were enriched for nonessential genes with upstream TATA elements, those displaying transient GEIs were enriched for essential genes regardless of TATA regulation. Genes subject to persistent GEI influences showed relaxed constraint on acclimated gene expression compared to the average yeast gene, whereas genes restricted to transient GEIs did not. We propose that transient GEI during the transition between environments provides a previously unappreciated source of expression variation, particularly for essential genes.
Related articles in Genetics:
ISSUE HIGHLIGHTS
Genetics 2010 184: NP.
 |