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Originally published as Genetics Published Articles Ahead of Print on November 30, 2009.

Genetics, Vol. 184, 351-361, February 2010, Copyright © 2010
doi:10.1534/genetics.109.111393

FRQ-Interacting RNA Helicase Mediates Negative and Positive Feedback in the Neurospora Circadian Clock

Mi Shi*,1, Michael Collett*,2, Jennifer J. Loros*,{dagger} and Jay C. Dunlap*,3

Departments of * Genetics and {dagger} Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755

3 Corresponding author: 7400 Remsen, Department of Genetics, Dartmouth Medical School, Hanover, NH 03755.
E-mail: Jay.C.Dunlap{at}dartmouth.edu

The Neurospora circadian oscillator comprises FREQUENCY (FRQ) and its transcription activator, the White Collar Complex (WCC). Repression of WCC's transcriptional activity by FRQ via negative feedback is indispensable for clock function. An unbiased genetic screen that targeted mutants with defects in negative feedback regulation yielded a fully viable arrhythmic strain bearing a novel allele of FRQ-interacting RNA helicase (frh), an essential gene that encodes a putative exosome component protein. In the allele, frhR806H, clock function is completely disturbed, while roles of FRQ-interacting RNA helicase (FRH) essential for viability are left intact. FRHR806H still interacts with FRQ, but interaction between the FRQ–FRHR806H complex (FFC) and WCC is severely affected. Phosphorylation of WC-1 is reduced in the mutant leading to constantly elevated WCC activity, which breaks the negative feedback loop. WCC levels are considerably reduced in the mutant, especially those of WC-1, consistent both with loss of positive feedback (FRQ-dependent WC-1 stabilization) and with a reduced level of the FRQ-mediated WCC phosphorylation that leads to high WCC activity accompanied by rapid transcription-associated turnover. FRH overexpression promotes WC-1 accumulation, confirming that FRH together with FRQ plays a role in WC-1 stabilization. Identification of a viable allele of frh, displaying virtually complete loss of both negative and positive circadian feedback, positions FRH as a core component of the central oscillator that is permissive for rhythmicity but appears not to modulate periodicity. Moreover, the results suggest that there are clock-specific roles for FRH that are distinct from the predicted essential exosome-associated functions for the protein.