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Originally published as Genetics Published Articles Ahead of Print on October 12, 2009.
Genetics, Vol. 183, 1591-1595, December 2009, Copyright © 2009
doi:10.1534/genetics.109.109041
Analysis of Ipl1-Mediated Phosphorylation of the Ndc80 Kinetochore Protein in Saccharomyces cerevisiae
Bungo Akiyoshi*,
,
Christian R. Nelson*,
Jeffrey A. Ranish
and
Sue Biggins*,1
* Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, Molecular and Cellular Biology Program, University of Washington, Seattle, Washington 98195 and Institute for Systems Biology, Seattle, Washington 98103
1 Corresponding author: 1100 Fairview Ave. N, A2-168, P. O. Box 19024, Seattle, WA 98109.
E-mail: sbiggins{at}fhcrc.org
Phosphorylation of the Ndc80 kinetochore protein by the Ipl1/Aurora B kinase reduces its microtubule binding activity in vitro. We found that kinetochore-bound Ndc80 is phosphorylated on Ipl1 sites in vivo, but this phosphorylation is not essential. Instead, we show that additional Ipl1 targets contribute to segregation and the spindle checkpoint.
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