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Originally published as Genetics Published Articles Ahead of Print on June 29, 2009.
Genetics, Vol. 183, 399-402, September 2009, Copyright © 2009
doi:10.1534/genetics.109.105213
Recombinase-Mediated Cassette Exchange Provides a Versatile Platform for Gene Targeting: Knockout of miR-31b
Ruifen Weng*,
,
Ya-Wen Chen*,
Natascha Bushati*,1,
Adam Cliffe*,2 and
Stephen M. Cohen*,,3
* Temasek Life Sciences Laboratory and Department of Biological Sciences, National University of Singapore, 117604, Republic of Singapore
3 Corresponding author: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore, 117604, Republic of Singapore.
E-mail: cohen{at}tll.org.sg
A series of vectors has been designed to enhance the versatility of targeted homologous recombination. Recombinase-mediated cassette exchange permits sequential targeting at any locus and improves flexibility in making user-defined mutations. Application of RMCE to delete an intronic microRNA gene is described.
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