Originally published as Genetics Published Articles Ahead of Print on May 27, 2009.

Genetics, Vol. 182, 1381-1385, August 2009, Copyright © 2009
doi:10.1534/genetics.109.104737

Molecular Isolation of the M Gene Suggests That a Conserved-Residue Conversion Induces the Formation of Bisexual Flowers in Cucumber Plants

* School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China, {dagger} Institute of Vegetables and Flowers, Chinese Academy of Agriculture Sciences, Beijing 100081, China, {ddagger} Hunan Vegetable Research Institute, Hunan Academy of Agriculture Sciences, Changsha 410125, China, § School of Horticulture, Shenyang Agriculture University, Shenyang 110161, China and ** Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

2 Corresponding author: Shanghai Jiaotong University, Dongchuan Rd., No. 800, Shanghai 200240, China.
E-mail: cairun{at}sjtu.edu.cn

Sex determination in plants involves a variety of mechanisms. Here, we report the map-based cloning and characterization of the unisexual-flower-controlling gene M. M was identified as a previously characterized putative 1-aminocyclopropane-1-carboxylic acid synthase gene, while the m allele that mutated at a conserved site (Gly33Cys) lost activity in the original enzymatically active allele.