Originally published as Genetics Published Articles Ahead of Print on March 30, 2009.

Genetics, Vol. 182, 623-626, June 2009, Copyright © 2009
doi:10.1534/genetics.109.103036

Precise Gene-Dose Alleles for Chemical Genetics

Zhun Yan^*,, Nicolas M. Berbenetz^,, Guri Giaever^*,, and Corey Nislow^,,,1

^* Department of Pharmaceutical Sciences, University of Toronto, Toronto, Ontario M5S 3M2, Canada, Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada, Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario M5G 1L6, Canada and Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada

¹ Corresponding author: University of Toronto, Donnelly Centre for Cellular and Biomolecular Research, Room 1210, 160 College St., Toronto, ON M5S 3E1, Canada.
E-mail: corey.nislow{at}gmail.com

Modulating gene dose is an effective way to alter protein levels and modify phenotypes to understand gene function. In addition, combining gene-dose alleles with chemical perturbation can provide insight into drug–gene interactions. Here, we present a strategy that combines diverse loss-of-function alleles to systematically modulate gene dose in Saccharomyces cerevisiae. The generated gene dosage allele set expands the genetic toolkit for uncovering novel phenotypes.

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Online ISSN: 1943-2361  Print ISSN: 0016-6731
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