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Originally published as Genetics Published Articles Ahead of Print on February 9, 2009.
Genetics, Vol. 181, 1687-1691, April 2009, Copyright © 2009
doi:10.1534/genetics.108.097600
Unexpected Expression Pattern of Tetracycline-Regulated Transgenes in Mice
M. Bao-Cutrona*,
,1 and
P. Moral*
* Department of Animal Biology-Anthropology, Faculty of Biology, University of Barcelona, 08028 Barcelona, Spain and Department of Neuroscience, Istituto di Ricerche Farmacologiche ‘Mario Negri’, 20156 Milan, Italy
1 Corresponding author: Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, Via Nazionale per Lanciano, 8, 66030 Santa Maria Imbaro (CH), Italy.
E-mail: meritxell{at}negrisud.it
In generating a conditional transgenic murine model based on a tetracycline-regulated system, we obtained unexpected patterns of expression due to the transcriptional inactivity of the tet-responder promoter. Here we show strong cell-type-restricted expression that was variegated to an extent determined by the number of responder transgene copies integrated into the host genome.
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