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Originally published as Genetics Published Articles Ahead of Print on October 28, 2008.
Genetics, Vol. 181, 335-340, January 2009, Copyright © 2009
doi:10.1534/genetics.108.093104
Drosophila Hold'em Is Required for a Subset of Meiotic Crossovers and Interacts With the DNA Repair Endonuclease Complex Subunits MEI-9 and ERCC1
Eric F. Joyce, S. Nikhila Tanneti and Kim S. McKim1
Waksman Institute and Department of Genetics, Rutgers, the State University of New Jersey, Piscataway, New Jersey 08854-8020
1 Corresponding author: Waksman Institute, Rutgers University, 190 Frelinghuysen Rd., Piscataway, NJ 08854.
E-mail: mckim{at}rci.rutgers.edu
Three Drosophila proteins, ERCC1, MUS312, and MEI-9, function in a complex proposed to resolve double-Holliday-junction intermediates into crossovers during meiosis. We report here the characterization of hold'em (hdm), whose protein product belongs to a single-strand-DNA-binding superfamily of proteins. Mutations in hdm result in reduced meiotic crossover formation and sensitivity to the DNA-damaging agent methyl methanesulfonate. Furthermore, HDM physically interacts with both MEI-9 and ERCC1 in a yeast two-hybrid assay. We conclude that HDM, MEI-9, MUS312, and ERCC1 form a complex that resolves meiotic recombination intermediates into crossovers.
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