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Originally published as Genetics Published Articles Ahead of Print on October 28, 2008.
Genetics, Vol. 181, 301-311, January 2009, Copyright © 2009
doi:10.1534/genetics.108.096636
LINE-Like Retrotransposition in Saccharomyces cerevisiae
Chun Dong*,
Russell T. Poulter
and
Jeffrey S. Han*,1
* Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland, 21218 and Department of Biochemistry, University of Otago, Dunedin 9054, New Zealand
1 Corresponding author: Carnegie Institution of Washington, 3520 San Martin Dr., Baltimore, MD 21218.
E-mail: han{at}ciwemb.edu
Over one-third of human genome sequence is a product of non-LTR retrotransposition. The retrotransposon that currently drives this process in humans is the highly abundant LINE-1 (L1) element. Despite the ubiquitous nature of L1's in mammals, we still lack a complete mechanistic understanding of the L1 replication cycle and how it is regulated. To generate a genetically amenable model for non-LTR retrotransposition, we have reengineered the Zorro3 retrotransposon, an L1 homolog from Candida albicans, for use in the budding yeast Saccharomyces cerevisiae. We found that S. cerevisiae, which has no endogenous L1 homologs or remnants, can still support Zorro3 retrotransposition. Analysis of Zorro3 mutants and insertion structures suggest that this is authentic L1-like retrotransposition with remarkable resemblance to mammalian L1-mediated events. This suggests that S. cerevisiae has unexpectedly retained the basal host machinery required for L1 retrotransposition. This model will also serve as a powerful system to study the cell biology of L1 elements and for the genetic identification and characterization of cellular factors involved in L1 retrotransposition.
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Genetics 2009 181: NP.
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