- THIS ARTICLE
- Full Text
- Full Text (PDF)
-
All Versions of this Article:
genetics.108.096891v1
181/1/153 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Email this article to a friend
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Hapiak, V. M.
- Articles by Komuniecki, R. W.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Hapiak, V. M.
- Articles by Komuniecki, R. W.
Originally published as Genetics Published Articles Ahead of Print on November 10, 2008.
Genetics, Vol. 181, 153-163, January 2009, Copyright © 2009
doi:10.1534/genetics.108.096891
Dual Excitatory and Inhibitory Serotonergic Inputs Modulate Egg Laying in Caenorhabditis elegans
Vera M. Hapiak, Robert J. Hobson1, Lindsay Hughes, Katherine Smith, Gareth Harris, Christina Condon, Patricia Komuniecki and Richard W. Komuniecki2
Department of Biological Sciences, University of Toledo, Toledo, Ohio 43606-3390
2 Corresponding author: Department of Biological Sciences, University of Toledo, 2801 W. Bancroft St., Toledo, OH 43606-3390.
E-mail: rkomuni{at}utnet.utoledo.edu
Serotonin (5-HT) regulates key processes in both vertebrates and invertebrates. Previously, four 5-HT receptors that contributed to the 5-HT modulation of egg laying were identified in Caenorhabditis elegans. Therefore, to assess potential receptor interactions, we generated animals containing combinations of null alleles for each receptor, especially animals expressing only individual 5-HT receptors. 5-HT-stimulated egg laying and egg retention correlated well with different combinations of predicted excitatory and inhibitory serotonergic inputs. For example, 5-HT did not stimulate egg laying in ser-1, ser-7, or ser-7 ser-1 null animals, and ser-7 ser-1 animals retained more eggs than wild-type animals. In contrast, 5-HT-stimulated egg laying in ser-4;mod-1 animals was greater than in wild-type animals, and ser-4;mod-1 animals retained fewer eggs than wild-type animals. Surprisingly, ser-4;mod-1;ser-7 ser-1 animals retained the same number of eggs as wild-type animals and exhibited significant 5-HT-stimulated egg laying that was dependent on a previously uncharacterized receptor, SER-5. 5-HT-stimulated egg laying was absent in ser-5;ser-4;mod-1;ser-7 ser-1 animals, and these animals retained more eggs than either wild-type or ser-4;mod-1;ser-7 ser-1 animals. The 5-HT sensitivity of egg laying could be restored by ser-5 muscle expression. Together, these results highlight the dual excitatory/inhibitory serotonergic inputs that combine to modulate egg laying.
This article has been cited by other articles:
 |
|
 |
|
  G. P. Harris, V. M. Hapiak, R. T. Wragg, S. B. Miller, L. J. Hughes, R. J. Hobson, R. Steven, B. Bamber, and R. W. Komuniecki Three Distinct Amine Receptors Operating at Different Levels within the Locomotory Circuit Are Each Essential for the Serotonergic Modulation of Chemosensation in Caenorhabditis elegans J. Neurosci., February 4, 2009; 29(5): 1446 - 1456. [Abstract] [Full Text] [PDF]
|
|
|