Originally published as Genetics Published Articles Ahead of Print on October 1, 2008.

Genetics, Vol. 180, 2019-2031, December 2008, Copyright © 2008
doi:10.1534/genetics.108.091512

Arabidopsis iba response5 Suppressors Separate Responses to Various Hormones

Lucia C. Strader, Melanie Monroe-Augustus, Kristen C. Rogers1, Grace L. Lin2 and Bonnie Bartel3

Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005

3 Corresponding author: Department of Biochemistry and Cell Biology, MS-140, Rice University, 6100 Main St., Houston, TX 77005.
E-mail: bartel{at}rice.edu

Auxin controls numerous plant growth processes by directing cell division and expansion. Auxin-response mutants, including iba response5 (ibr5), exhibit a long root and decreased lateral root production in response to exogenous auxins. ibr5 also displays resistance to the phytohormone abscisic acid (ABA). We found that the sar3 suppressor of auxin resistant1 (axr1) mutant does not suppress ibr5 auxin-response defects, suggesting that screening for ibr5 suppressors might reveal new components important for phytohormone responsiveness. We identified two classes of Arabidopsis thaliana mutants that suppressed ibr5 resistance to indole-3-butyric acid (IBA): those with restored responses to both the auxin precursor IBA and the active auxin indole-3-acetic acid (IAA) and those with restored response to IBA but not IAA. Restored IAA sensitivity was accompanied by restored ABA responsiveness, whereas suppressors that remained IAA resistant also remained ABA resistant. Some suppressors restored sensitivity to both natural and synthetic auxins; others restored responsiveness only to auxin precursors. We used positional information to determine that one ibr5 suppressor carried a mutation in PLEIOTROPIC DRUG RESISTANCE9 (PDR9/ABCG37/At3g53480), which encodes an ATP-binding cassette transporter previously implicated in cellular efflux of the synthetic auxin 2,4-dichlorophenoxyacetic acid.