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Originally published as Genetics Published Articles Ahead of Print on September 14, 2008.
Genetics, Vol. 180, 1763-1766, November 2008, Copyright © 2008
doi:10.1534/genetics.108.094508
A Simple Polymerase Chain Reaction-Based Method for the Construction of Recombinase-Mediated Cassette Exchange Donor Vectors
Jack R. Bateman1 and C.-ting Wu
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
1 Corresponding author: Department of Biology, Bowdoin College, 6500 College Station, Brunswick, ME 04011.
E-mail: jbateman{at}bowdoin.edu
Here we describe a simple method for generating donor vectors suitable for targeted transgenesis via recombinase-mediated cassette exchange (RMCE) using the 
C31 integrase. This PCR-based strategy employs small attB "tails" on the primers used to amplify a sequence of interest, permitting the rapid creation of transgenes for in vivo analysis.
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