- THIS ARTICLE
- Full Text
- Full Text (PDF)
- Data Supplement
-
All Versions of this Article:
genetics.108.093211v1
180/3/1319 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Email this article to a friend
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Tang, X.
- Articles by Bai, Y.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Tang, X.
- Articles by Bai, Y.
Originally published as Genetics Published Articles Ahead of Print on September 14, 2008.
Genetics, Vol. 180, 1319-1328, November 2008, Copyright © 2008
doi:10.1534/genetics.108.093211
Cross-Species Bacterial Artificial Chromosome–Fluorescence in Situ Hybridization Painting of the Tomato and Potato Chromosome 6 Reveals Undescribed Chromosomal Rearrangements
Xiaomin Tang*,1,
Dóra Szinay
,1,
Chunting Lang,
Munikote S. Ramanna*,
Edwin A. G. van der Vossen*,
Erwin Datema
,
René Klein Lankhorst,
Jan de Boer*,
Sander A. Peters,
Christian Bachem*,
Willem Stiekema,
Richard G. F. Visser*,
Hans de Jong and
Yuling Bai*,2
* Wageningen-UR Plant Breeding, 6708 PB Wageningen, The Netherlands, Laboratory of Genetics, Wageningen University, 6703 BD Wageningen, The Netherlands and Centre for Biosystems Genomics, 6708 PB Wageningen, The Netherlands
2 Corresponding author: Wageningen-UR Plant Breeding, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
E-mail: bai.yuling{at}wur.nl
Ongoing genomics projects of tomato (Solanum lycopersicum) and potato (S. tuberosum) are providing unique tools for comparative mapping studies in Solanaceae. At the chromosomal level, bacterial artificial chromosomes (BACs) can be positioned on pachytene complements by fluorescence in situ hybridization (FISH) on homeologous chromosomes of related species. Here we present results of such a cross-species multicolor cytogenetic mapping of tomato BACs on potato chromosomes 6 and vice versa. The experiments were performed under low hybridization stringency, while blocking with Cot-100 was essential in suppressing excessive hybridization of repeat signals in both within-species FISH and cross-species FISH of tomato BACs. In the short arm we detected a large paracentric inversion that covers the whole euchromatin part with breakpoints close to the telomeric heterochromatin and at the border of the short arm pericentromere. The long arm BACs revealed no deviation in the colinearity between tomato and potato. Further comparison between tomato cultivars Cherry VFNT and Heinz 1706 revealed colinearity of the tested tomato BACs, whereas one of the six potato clones (RH98-856-18) showed minor putative rearrangements within the inversion. Our results present cross-species multicolor BAC–FISH as a unique tool for comparative genetic studies across Solanum species.
This article has been cited by other articles:
 |
|
 |
|
  L. A. Mueller, R. K. Lankhorst, S. D. Tanksley, J. J. Giovannoni, R. White, J. Vrebalov, Z. Fei, J. van Eck, R. Buels, A. A. Mills, et al. A Snapshot of the Emerging Tomato Genome Sequence The Plant Genome, March 1, 2009; 2(1): 78 - 92. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Iovene, S. M. Wielgus, P. W. Simon, C. R. Buell, and J. Jiang Chromatin Structure and Physical Mapping of Chromosome 6 of Potato and Comparative Analyses With Tomato Genetics, November 1, 2008; 180(3): 1307 - 1317. [Abstract] [Full Text] [PDF]
|
|