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Originally published as Genetics Published Articles Ahead of Print on September 14, 2008.
Genetics, Vol. 180, 1307-1317, November 2008, Copyright © 2008
doi:10.1534/genetics.108.093179
Chromatin Structure and Physical Mapping of Chromosome 6 of Potato and Comparative Analyses With Tomato
Marina Iovene*,1,
Susan M. Wielgus*,
Philipp W. Simon
,
C. Robin Buell
and
Jiming Jiang*,2
U.S. Department of Agriculture–Agricultural Research Service, Vegetable Crops Unit, * Department of Horticulture, University of Wisconsin, Madison, Wisconsin 53706 and Department of Plant Biology, Michigan State University, East Lansing, Michigan 48824
2 Corresponding author: Department of Horticulture, University of Wisconsin, 1575 Linden Dr., Madison, WI 53706.
E-mail: jjiang1{at}wisc.edu
Potato (Solanum tuberosum) has the densest genetic linkage map and one of the earliest established cytogenetic maps among all plant species. However, there has been limited effort to integrate these maps. Here, we report fluorescence in situ hybridization (FISH) mapping of 30 genetic marker-anchored bacterial artificial chromosome (BAC) clones on the pachytene chromosome 6 of potato. The FISH mapping results allowed us to define the genetic positions of the centromere and the pericentromeric heterochromatin and to relate chromatin structure to the distribution of recombination along the chromosome. A drastic reduction of recombination was associated with the pericentromeric heterochromatin that accounts for 
28% of the physical length of the pachytene chromosome. The pachytene chromosomes 6 of potato and tomato (S. lycopersicum) share a similar morphology. However, distinct differences of heterochromatin distribution were observed between the two chromosomes. FISH mapping of several potato BACs on tomato pachytene chromosome 6 revealed an overall colinearity between the two chromosomes. A chromosome inversion was observed in the euchromatic region of the short arms. These results show that the potato and tomato genomes contain more chromosomal rearrangements than those reported previously on the basis of comparative genetic linkage mapping.
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