- THIS ARTICLE
- Full Text
- Full Text (PDF)
- Data Supplement
-
All Versions of this Article:
genetics.108.090563v1
180/1/703 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Email this article to a friend
- Related articles in Genetics
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Huang, J.
- Articles by Hong, Y.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Huang, J.
- Articles by Hong, Y.
Originally published as Genetics Published Articles Ahead of Print on August 30, 2008.
Genetics, Vol. 180, 703-707, September 2008, Copyright © 2008
doi:10.1534/genetics.108.090563
Efficient Ends-Out Gene Targeting In Drosophila
Juan Huang*,1,
Wenke Zhou*,1,
Annie M. Watson*,
Yuh-Nung Jan
and
Yang Hong*,2
* Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennyslvania 15261 and Howard Hughes Medical Institute, Departments of Physiology and Biochemistry, University of California, San Francisco, California 94143
2 Corresponding author: Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, S325 BST, 3500 Terrace St., Pittsburgh, PA 15261.
E-mail: yhong{at}pitt.edu
In this report, we describe several approaches to improve the scalability and throughput of major genetic crosses in ends-out gene targeting. We generated new sets of targeting vectors and fly stocks and introduced a novel negative selection marker that drastically reduced the frequency of false-positive targeting candidates.
Related articles in Genetics:
ISSUE HIGHLIGHTS
Genetics 2008 180: NP.
This article has been cited by other articles:
 |
|
 |
|
  J. Huang, W. Zhou, W. Dong, A. M. Watson, and Y. Hong From the Cover: Directed, efficient, and versatile modifications of the Drosophila genome by genomic engineering PNAS, May 19, 2009; 106(20): 8284 - 8289. [Abstract] [Full Text] [PDF]
|
|