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Originally published as Genetics Published Articles Ahead of Print on August 30, 2008.
Genetics, Vol. 180, 673-679, September 2008, Copyright © 2008
doi:10.1534/genetics.108.089698
Gene Conversion and End-Joining-Repair Double-Strand Breaks in the Caenorhabditis elegans Germline
Valérie J. Robert*,
,
M. Wayne Davis
,
Erik M. Jorgensen and
Jean-Louis Bessereau*,,1
* Ecole Normale Supérieure, Biologie Cellulaire de la Synapse, Paris, F-75005 France, Inserm, U789, Paris, F-75005 France and Howard Hughes Medical Institute and the Department of Biology, University of Utah, Salt Lake City, Utah 84112-0840
1 Corresponding author: ENS, INSERM U789, 46 Rue d'Ulm, 75005 Paris, France.
E-mail: jlbesse{at}biologie.ens.fr
Excision of a Mos1 transposon in the germline of Caenorhabditis elegans generates a double-strand break in the chromosome. We demonstrate that breaks are most prominently repaired by gene conversion from the homolog, but also rarely by nonhomologous end-joining. In some cases, gene conversion events are resolved by crossing over. Surprisingly, expression of the transposase using an intestine-specific promoter can induce repair, raising the possibility that activation of transposase expression in somatic cells can lead to transposition of Mos1 in the germline.