Originally published as Genetics Published Articles Ahead of Print on August 9, 2008.

Genetics, Vol. 179, 2195-2211, August 2008, Copyright © 2008
doi:10.1534/genetics.107.071332

Experimental Estimation of Mutation Rates in a Wheat Population With a Gene Genealogy Approach

Anne-Laure Raquin*, Frantz Depaulis{dagger}, Amaury Lambert{dagger}, Nathalie Galic*, Philippe Brabant* and Isabelle Goldringer*,1

* UMR de Génétique Végétale, INRA, CNRS, Université Paris Sud, AgroParisTech, Ferme du Moulon, F91190 Gif-sur-Yvette, France and {dagger} Laboratoire d'Ecologie, CNRS, UMR 7625, Ecole Normale Supérieure, 75230 Paris Cedex 05, France

1 Corresponding author: UMR de Génétique Végétale, INRA/CNRS/Université Paris Sud/AgroParisTech, Ferme du Moulon, 91190 Gif-sur-Yvette, France.
E-mail: isa{at}moulon.inra.fr

Microsatellite markers are extensively used to evaluate genetic diversity in natural or experimental evolving populations. Their high degree of polymorphism reflects their high mutation rates. Estimates of the mutation rates are therefore necessary when characterizing diversity in populations. As a complement to the classical experimental designs, we propose to use experimental populations, where the initial state is entirely known and some intermediate states have been thoroughly surveyed, thus providing a short timescale estimation together with a large number of cumulated meioses. In this article, we derived four original gene genealogy-based methods to assess mutation rates with limited bias due to relevant model assumptions incorporating the initial state, the number of new alleles, and the genetic effective population size. We studied the evolution of genetic diversity at 21 microsatellite markers, after 15 generations in an experimental wheat population. Compared to the parents, 23 new alleles were found in generation 15 at 9 of the 21 loci studied. We provide evidence that they arose by mutation. Corresponding estimates of the mutation rates ranged from 0 to 4.97 x 10–3 per generation (i.e., year). Sequences of several alleles revealed that length polymorphism was only due to variation in the core of the microsatellite. Among different microsatellite characteristics, both the motif repeat number and an independent estimation of the Nei diversity were correlated with the novel diversity. Despite a reduced genetic effective size, global diversity at microsatellite markers increased in this population, suggesting that microsatellite diversity should be used with caution as an indicator in biodiversity conservation issues.