- THIS ARTICLE
- Full Text
- Full Text (PDF)
-
All Versions of this Article:
genetics.108.088963v1
179/4/1957 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Email this article to a friend
- Related articles in Genetics
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Shakir, M. A.
- Articles by Lundquist, E. A.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Shakir, M. A.
- Articles by Lundquist, E. A.
Originally published as Genetics Published Articles Ahead of Print on August 9, 2008.
Genetics, Vol. 179, 1957-1971, August 2008, Copyright © 2008
doi:10.1534/genetics.108.088963
The Arp2/3 Activators WAVE and WASP Have Distinct Genetic Interactions With Rac GTPases in Caenorhabditis elegans Axon Guidance
M. Afaq Shakir*,1,2,
Ke Jiang*,1,3,
Eric C. Struckhoff*,
Rafael S. Demarco*,
Falshruti B. Patel
,
Martha C. Soto and
Erik A. Lundquist*,4
* Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas 66045-7534 and Department of Pathology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854
4 Corresponding author: Department of Molecular Biosciences, 1200 Sunnyside Ave., 5049 Haworth Hall, University of Kansas, Lawrence, KS 66045.
E-mail: erikl{at}ku.edu
In the developing nervous system, axons are guided to their targets by the growth cone. Lamellipodial and filopodial protrusions from the growth cone underlie motility and guidance. Many molecules that control lamellipodia and filopodia formation, actin organization, and axon guidance have been identified, but it remains unclear how these molecules act together to control these events. Experiments are described here that indicate that, in Caenorhabditis elegans, two WH2-domain-containing activators of the Arp2/3 complex, WVE-1/WAVE and WSP-1/WASP, act redundantly in axon guidance and that GEX-2/Sra-1 and GEX-3/Kette, molecules that control WAVE activity, might act in both pathways. WAVE activity is controlled by Rac GTPases, and data are presented here that suggest WVE-1/WAVE and CED-10/Rac act in parallel to a pathway containing WSP-1/WASP and MIG-2/RhoG. Furthermore, results here show that the CED-10/WVE-1 and MIG-2/WSP-1 pathways act in parallel to two other molecules known to control lamellipodia and filopodia and actin organization, UNC-115/abLIM and UNC-34/Enabled. These results indicate that at least three actin-modulating pathways act in parallel to control actin dynamics and lamellipodia and filopodia formation during axon guidance (WASP–WAVE, UNC-115/abLIM, and UNC-34/Enabled).
Related articles in Genetics:
ISSUE HIGHLIGHTS
Genetics 2008 179: NP.
This article has been cited by other articles:
 |
|
 |
|
  M. Alexander, K. K. M. Chan, A. B. Byrne, G. Selman, T. Lee, J. Ono, E. Wong, R. Puckrin, S. J. Dixon, and P. J. Roy An UNC-40 pathway directs postsynaptic membrane extension in Caenorhabditis elegans Development, March 15, 2009; 136(6): 911 - 922. [Abstract] [Full Text] [PDF]
|
|