Genetics, Vol. 179, 1795-1806, August 2008, Copyright © 2008
doi:10.1534/genetics.108.089821

Mutagenic and Recombinagenic Responses to Defective DNA Polymerase Are Facilitated by the Rev1 Protein in pol3-t Mutants of Saccharomyces cerevisiae

Erica Mito^*,1, Janet V. Mokhnatkin^*,, Molly C. Steele^*,, Victoria L. Buettner, Steve S. Sommer, Glenn M. Manthey^* and Adam M. Bailis^*,2

^* Division of Molecular Biology, Beckman Research Institute, City of Hope National Medical Center, Duarte, California 91010-3000, Dual Admissions Program, Miami University of Ohio and University of Cincinnati College of Medicine, Oxford, Ohio 45056, Program in Biological Sciences, University of Chicago, Chicago, Illinois 60637 and Department of Molecular Genetics, City of Hope National Medical Center, Duarte, California 91010-3000

² Corresponding author: Division of Molecular Biology, Beckman Research Institute, City of Hope National Medical Center, 1500 East Duarte Rd., Duarte, CA 91010.
E-mail: abailis{at}bricoh.edu

Defective DNA replication can result in substantial increases in the level of genome instability. In the yeast Saccharomyces cerevisiae, the pol3-t allele confers a defect in the catalytic subunit of replicative DNA polymerase that results in increased rates of mutagenesis, recombination, and chromosome loss, perhaps by increasing the rate of replicative polymerase failure. The translesion polymerases Pol , Pol , and Rev1 are part of a suite of factors in yeast that can act at sites of replicative polymerase failure. While mutants defective in the translesion polymerases alone displayed few defects, loss of Rev1 was found to suppress the increased rates of spontaneous mutation, recombination, and chromosome loss observed in pol3-t mutants. These results suggest that Rev1 may be involved in facilitating mutagenic and recombinagenic responses to the failure of Pol . Genome stability, therefore, may reflect a dynamic relationship between primary and auxiliary DNA polymerases.

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