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Originally published as Genetics Published Articles Ahead of Print on May 27, 2008.
Genetics, Vol. 179, 773-784, June 2008, Copyright © 2008
doi:10.1534/genetics.107.077727
Sites of Recombination Are Local Determinants of Meiotic Homolog Pairing in Saccharomyces cerevisiae
Joshua Chang Mell*,
,
Bethany L. Wienholz,
Asmaa Salem and
Sean M. Burgess*,,1
* Department of Molecular and Cellular Biology, Genetics Graduate Group, University of California, Davis, California 95616
1 Corresponding author: Section of Molecular and Cellular Biology, Briggs Hall, University of California, One Shields Ave., Davis, CA 95616.
E-mail: smburgess{at}ucdavis.edu
Trans-acting factors involved in the early meiotic recombination pathway play a major role in promoting homolog pairing during meiosis in many plants, fungi, and mammals. Here we address whether or not allelic sites have higher levels of interaction when in cis to meiotic recombination events in the budding yeast Saccharomyces cerevisiae. We used Cre/loxP site-specific recombination to genetically measure the magnitude of physical interaction between loxP sites located at allelic positions on homologous chromosomes during meiosis. We observed nonrandom coincidence of Cre-mediated loxP recombination events and meiotic recombination events when the two occurred at linked positions. Further experiments showed that a subset of recombination events destined to become crossover products increased the frequency of nearby Cre-mediated loxP recombination. Our results support a simple physical model of homolog pairing in budding yeast, where recombination at numerous genomic positions generally serves to loosely coalign homologous chromosomes, while crossover-bound recombination intermediates locally stabilize interactions between allelic sites.
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Genetics 2008 179: NP.
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  J. C. Mell, K. Komachi, O. Hughes, and S. Burgess Cooperative Interactions Between Pairs of Homologous Chromatids During Meiosis in Saccharomyces cerevisiae Genetics, June 1, 2008; 179(2): 1125 - 1127. [Abstract] [Full Text] [PDF]
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