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Originally published as Genetics Published Articles Ahead of Print on February 1, 2008.
Genetics, Vol. 178, 801-814, February 2008, Copyright © 2008
doi:10.1534/genetics.107.081588
Caenorhabditis elegans ABCRNAi Transporters Interact Genetically With rde-2 and mut-7
Prema Sundaram1, Wang Han, Nancy Cohen, Benjamin Echalier2, John Albin3 and Lisa Timmons4
Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas 66045
4 Corresponding author: Department of Molecular Biosciences, 5041 Haworth Hall, 1200 Sunnyside Ave., University of Kansas, Lawrence, KS 66045.
E-mail: timmons{at}ku.edu
RNA interference (RNAi) mechanisms are conserved and consist of an interrelated network of activities that not only respond to exogenous dsRNA, but also perform endogenous functions required in the fine tuning of gene expression and in maintaining genome integrity. Not surprisingly, RNAi functions have widespread influences on cellular function and organismal development. Previously, we observed a reduced capacity to mount an RNAi response in nine Caenorhabditis elegans mutants that are defective in ABC transporter genes (ABCRNAi mutants). Here, we report an exhaustive study of mutants, collectively defective in 49 different ABC transporter genes, that allowed for the categorization of one additional transporter into the ABCRNAi gene class. Genetic complementation tests reveal functions for ABCRNAi transporters in the mut-7/rde-2 branch of the RNAi pathway. These second-site noncomplementation interactions suggest that ABCRNAi proteins and MUT-7/RDE-2 function together in parallel pathways and/or as multiprotein complexes. Like mut-7 and rde-2, some ABCRNAi mutants display transposon silencing defects. Finally, our analyses reveal a genetic interaction network of ABCRNAi gene function with respect to this part of the RNAi pathway. From our results, we speculate that the coordinated activities of ABCRNAi transporters, through their effects on endogenous RNAi-related mechanisms, ultimately affect chromosome function and integrity.
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Genetics 2008 178: NP.
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