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Genetics, Vol. 178, 633-647, February 2008, Copyright © 2008
doi:10.1534/genetics.107.082818
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, Contributes to the Fidelity of DNA Replication in Saccharomyces cerevisiae


* Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland and
Braun Laboratories, California Institute of Technology, Pasadena, California 91125
1 Corresponding author: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106 Warsaw, Poland.
E-mail: iwonaf{at}ibb.waw.pl
5' proofreading exonuclease (pol2-4) or to mutants defective in mismatch repair (msh6). The dpb2 pol2-4 and dpb2 msh6 double mutants show a synergistic increase in mutation rate, indicating that the mutations arising in the dpb2 mutants are due to DNA replication errors normally corrected by mismatch repair. The dpb2 mutations decrease the affinity of Dpb2p for the Pol2p subunit as measured by two-hybrid analysis, providing a possible mechanistic explanation for the loss of high-fidelity synthesis. Our results show that DNA polymerase subunits other than those housing the DNA polymerase and 3'
5' exonuclease are essential in controlling the level of spontaneous mutagenesis and genetic stability in yeast cells.
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