help button home button Genetics JPET
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Genetics, Vol. 178, 67-82, January 2008, Copyright © 2008
doi:10.1534/genetics.107.071506

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Data Supplement
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Related articles in Genetics
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lang, G. I.
Right arrow Articles by Murray, A. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lang, G. I.
Right arrow Articles by Murray, A. W.

Estimating the Per-Base-Pair Mutation Rate in the Yeast Saccharomyces cerevisiae

Gregory I. Lang and Andrew W. Murray1

Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138

1 Corresponding author: Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Ave., Room 3000, Cambridge, MA 02138.
E-mail: amurray{at}mcb.harvard.edu

Although mutation rates are a key determinant of the rate of evolution they are difficult to measure precisely and global mutations rates (mutations per genome per generation) are often extrapolated from the per-base-pair mutation rate assuming that mutation rate is uniform across the genome. Using budding yeast, we describe an improved method for the accurate calculation of mutation rates based on the fluctuation assay. Our analysis suggests that the per-base-pair mutation rates at two genes differ significantly (3.80 x 10–10 at URA3 and 6.44 x 10–10 at CAN1) and we propose a definition for the effective target size of genes (the probability that a mutation inactivates the gene) that acknowledges that the mutation rate is nonuniform across the genome.


Related articles in Genetics:

ISSUE HIGHLIGHTS

Genetics 2008 178: NP. [Full Text]  



This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Lynch, W. Sung, K. Morris, N. Coffey, C. R. Landry, E. B. Dopman, W. J. Dickinson, K. Okamoto, S. Kulkarni, D. L. Hartl, et al.
From the Cover: A genome-wide view of the spectrum of spontaneous mutations in yeast
PNAS, July 8, 2008; 105(27): 9272 - 9277.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2008 by the Genetics Society of America.