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Genetics, Vol. 178, 593-596, January 2008, Copyright © 2008
doi:10.1534/genetics.107.079384
Characterization of Interactions Between and Among Components of the Meiotic Silencing by Unpaired DNA Machinery in Neurospora crassa Using Bimolecular Fluorescence Complementation
Nirmala Bardiya*,
William G. Alexander*,
Tony D. Perdue
,
Edward G. Barry,
Robert L. Metzenberg
,
Patricia J. Pukkila and
Patrick K. T. Shiu*,1
* Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211, Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599 and Department of Biology, California State University, Northridge, California 91330
1 Corresponding author: Division of Biological Sciences, University of Missouri, 103 Tucker Hall, Columbia, MO 65211.
E-mail: shiup{at}missouri.edu
Bimolecular fluorescence complementation (BiFC) is based on the complementation between two nonfluorescent fragments of the yellow fluorescent protein (YFP) when they are united by interactions between proteins covalently linked to them. We have successfully applied BiFC in Neurospora crassa using two genes involved in meiotic silencing by unpaired DNA (MSUD) and observed macromolecular complex formation involving only SAD-1 proteins, only SAD-2 proteins, and mixtures of SAD-1 and SAD-2 proteins.