- THIS ARTICLE
- Full Text
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Email this article to a friend
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Bardiya, N.
- Articles by Shiu, P. K. T.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Bardiya, N.
- Articles by Shiu, P. K. T.
Genetics, Vol. 178, 593-596, January 2008, Copyright © 2008
doi:10.1534/genetics.107.079384
Characterization of Interactions Between and Among Components of the Meiotic Silencing by Unpaired DNA Machinery in Neurospora crassa Using Bimolecular Fluorescence Complementation
Nirmala Bardiya*,
William G. Alexander*,
Tony D. Perdue
,
Edward G. Barry,
Robert L. Metzenberg
,
Patricia J. Pukkila and
Patrick K. T. Shiu*,1
* Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211, Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599 and Department of Biology, California State University, Northridge, California 91330
1 Corresponding author: Division of Biological Sciences, University of Missouri, 103 Tucker Hall, Columbia, MO 65211.
E-mail: shiup{at}missouri.edu
Bimolecular fluorescence complementation (BiFC) is based on the complementation between two nonfluorescent fragments of the yellow fluorescent protein (YFP) when they are united by interactions between proteins covalently linked to them. We have successfully applied BiFC in Neurospora crassa using two genes involved in meiotic silencing by unpaired DNA (MSUD) and observed macromolecular complex formation involving only SAD-1 proteins, only SAD-2 proteins, and mixtures of SAD-1 and SAD-2 proteins.
This article has been cited by other articles:
 |
|
 |
|
  S. Honda and E. U. Selker Tools for Fungal Proteomics: Multifunctional Neurospora Vectors for Gene Replacement, Protein Expression and Protein Purification Genetics, May 1, 2009; 182(1): 11 - 23. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Fleissner, S. Diamond, and N. L. Glass The Saccharomyces cerevisiae PRM1 Homolog in Neurospora crassa Is Involved in Vegetative and Sexual Cell Fusion Events but Also Has Postfertilization Functions Genetics, February 1, 2009; 181(2): 497 - 510. [Abstract] [Full Text] [PDF]
|
|
|