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Genetics, Vol. 178, 307-323, January 2008, Copyright © 2008
doi:10.1534/genetics.107.081869
A Gain-of-Function Suppressor Screen for Genes Involved in Dorsal–Ventral Boundary Formation in the Drosophila Wing
Fernando Bejarano1, Carlos M. Luque1,2, Héctor Herranz, Georgina Sorrosal, Neus Rafel, Thu Thuy Pham and Marco Milán3
Institució Catalana de Recerca i Estudis Avançats and Institute for Research in Biomedicine, 08028 Barcelona, Spain
3 Corresponding author: ICREA and Institute for Research in Biomedicine (IRB), Josep Samitier, 1-5, 08028 Barcelona, Spain.
E-mail: mmilan{at}pcb.ub.es
The Drosophila wing primordium is subdivided into a dorsal (D) and a ventral (V) compartment by the activity of the LIM-homeodomain protein Apterous in D cells. Cell interactions between D and V cells induce the activation of Notch at the DV boundary. Notch is required for the maintenance of the compartment boundary and the growth of the wing primordium. Beadex, a gain-of-function allele of dLMO, results in increased levels of dLMO protein, which interferes with the activity of Apterous and results in defects in DV axis formation. We performed a gain-of-function enhancer-promoter (EP) screen to search for suppressors of Beadex when overexpressed in D cells. We identified 53 lines corresponding to 35 genes. Loci encoding for micro-RNAs and proteins involved in chromatin organization, transcriptional control, and vesicle trafficking were characterized in the context of dLMO activity and DV boundary formation. Our results indicate that a gain-of-function genetic screen in a sensitized background, as opposed to classical loss-of-function-based screenings, is a very efficient way to identify redundant genes involved in a developmental process.
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