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Originally published as Genetics Published Articles Ahead of Print on October 18, 2007.
Genetics, Vol. 177, 2519-2523, December 2007, Copyright © 2007
doi:10.1534/genetics.107.081075
The Hermes Transposon of Musca domestica Is an Efficient Tool for the Mutagenesis of Schizosaccharomyces pombe
Adam G. Evertts*,
Christopher Plymire*,1,
Nancy L. Craig
and
Henry L. Levin*,2
* Section on Eukaryotic Transposable Elements, Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 and Department of Molecular Biology and Genetics, Howard Hughes Medical Institute and Johns Hopkins School of Medicine, Baltimore, Maryland 21205
2 Corresponding author: National Institutes of Health, Bldg. 18T, Room 106, Bethesda, MD 20892-5431.
E-mail: henry_levin{at}nih.gov
Currently, no transposon-based method for the mutagenesis of Schizosaccharomyces pombe exists. We have developed such a system based on the introduction of the hermes transposon from the housefly into S. pombe. This system efficiently disrupts open reading frames and allows the insertion sites to be readily identified.
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Genetics 2007 177: NP.