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Genetics, Vol. 177, 2457-2467, December 2007, Copyright © 2007
doi:10.1534/genetics.107.075416
A Microsatellite Genetic Map of the Turbot (Scophthalmus maximus)
Carmen Bouza*,
Miguel Hermida*,
Belén G. Pardo*,
Carlos Fernández*,
Gloria G. Fortes*,
Jaime Castro*,
Laura Sánchez*,
Pablo Presa
,
Montse Pérez,
Andrés Sanjuán,
Alejandro de Carlos,
José Antonio Álvarez-Dios
,
Susana Ezcurra
,
Rosa M. Cal**,
Francesc Piferrer and
Paulino Martínez*,1
* Department of Genetics, University of Santiago de Compostela, 27002 Lugo, Spain, Department of Biochemistry, Genetics and Inmunology, University of Vigo, 36310 Vigo, Spain, Department of Applied Mathematics, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain, R+D Stolt Sea Farm, Lira, 15292 Carnota, A Coruña, Spain, ** Spanish Oceanographic Institute, Oceanographic Center of Vigo, 36280 Vigo, Spain and Institute of Marine Science, Scientific Research Major Center, 08003 Barcelona, Spain
1 Corresponding author: Department of Genetics, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, Spain.
E-mail: paumarpo{at}lugo.usc.es
A consensus microsatellite-based linkage map of the turbot (Scophthalmus maximus) was constructed from two unrelated families. The mapping panel was derived from a gynogenetic family of 96 haploid embryos and a biparental diploid family of 85 full-sib progeny with known linkage phase. A total of 242 microsatellites were mapped in 26 linkage groups, six markers remaining unlinked. The consensus map length was 1343.2 cM, with an average distance between markers of 6.5 ± 0.5 cM. Similar length of female and male maps was evidenced. However, the mean recombination at common intervals throughout the genome revealed significant differences between sexes, 
1.6 times higher in the female than in the male. The comparison of turbot microsatellite flanking sequences against the Tetraodon nigroviridis genome revealed 55 significant matches, with a mean length of 102 bp and high sequence similarity (81–100%). The comparative mapping revealed significant syntenic regions among fish species. This study represents the first linkage map in the turbot, one of the most important flatfish in European aquaculture. This map will be suitable for QTL identification of productive traits in this species and for further evolutionary studies in fish and vertebrate species.
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  P. Martinez, C. Bouza, M. Hermida, J. Fernandez, M. A. Toro, M. Vera, B. Pardo, A. Millan, C. Fernandez, R. Vilas, et al. Identification of the Major Sex-Determining Region of Turbot (Scophthalmus maximus) Genetics, December 1, 2009; 183(4): 1443 - 1452. [Abstract] [Full Text] [PDF]
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