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Originally published as Genetics Published Articles Ahead of Print on October 18, 2007.
Genetics, Vol. 177, 2321-2333, December 2007, Copyright © 2007
doi:10.1534/genetics.107.078006
Interspecific Recombinant Congenic Strains Between C57BL/6 and Mice of the Mus spretus Species: A Powerful Tool to Dissect Genetic Control of Complex Traits
Gaëtan Burgio*,
,
Marek Szatanik
,
Jean-Louis Guénet*,
Maria-Rosa Arnau
,
Jean-Jacques Panthier* and
Xavier Montagutelli*,1
* Unité Postulante de Génétique Fonctionnelle de la Souris, CNRS URA 2578 and Unité des Neisseria, Institut Pasteur, 75015 Paris, France, Unité d'Éco-Anthropologie, Équipe "Génétique des Populations Humaines," CNRS MNHN, P7, UMR 5145, Musée de l'Homme, 75016 Paris, France and Universidad de la Laguna, 38207 Teneriffe, Spain
1 Corresponding author: Unité Postulante de Génétique Fonctionnelle de la Souris, 25 Rue du Docteur Roux, 75724 Paris Cedex 15, France.
E-mail: xmonta{at}pasteur.fr
Complex traits are under the genetic control of multiple genes, often with weak effects and strong epistatic interactions. We developed two new collections of mouse strains to improve genetic dissection of complex traits. They are derived from several backcrosses of the Mus spretus SEG/Pas or STF/Pas strains on the C57BL/6J background. Each of the 55 interspecific recombinant congenic strains (IRCSs) carries up to eight SEG/Pas chromosomal segments with an average size of 11.7 Mb, totalizing 1.37% of the genome. The complete series covers 39.7% of the SEG/Pas genome. As a complementary resource, six partial or complete interspecific consomic strains were developed and increased genome coverage to 45.6%. To evaluate the usefulness of these strains for QTL mapping, 16 IRCSs were compared with C57BL/6J for seven hematological parameters. Strain 66H, which carries three SEG/Pas chromosomal segments, had lower red blood cell volume and higher platelet count than C57BL/6J. Each chromosomal segment was isolated in a congenic strain to evaluate individual effects. Congenic strains were combined to assess epistasis. Our data show that both traits were controlled by several genes with complex epistatic interactions. IRCSs are therefore useful to unravel QTL with small effects and gene-by-gene interactions.
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