Originally published as Genetics Published Articles Ahead of Print on August 24, 2007.

Genetics, Vol. 177, 1227-1231, October 2007, Copyright © 2007
doi:10.1534/genetics.107.079293

A Single Nucleotide Polymorphism in the DNA Polymerase Gamma Gene of Saccharomyces cerevisiae Laboratory Strains Is Responsible for Increased Mitochondrial DNA Mutability

Enrico Baruffini^*,, Tiziana Lodi, Cristina Dallabona and Françoise Foury^*,1

^* Unité de Biochimie Physiologique, Université Catholique de Louvain, 1348 Louvain-la-Neuve, Belgium and Department of Genetics, Biology of Microorganisms, Anthropology, Evolution, University of Parma, 43100 Parma, Italy

¹ Corresponding author: Unité de Biochimie Physiologique, Croix du Sud 5-15, 1348 Louvain-la-Neuve, Belgium.
E-mail: foury{at}fysa.ucl.ac.be

In the Saccharomyces cerevisiae strains used for genome sequencing and functional analysis, the mitochondrial DNA replicase Mip1p contains a single nucleotide polymorphism changing the strictly conserved threonine 661 to alanine. This substitution is responsible for the increased rate of mitochondrial DNA point mutations and deletions in these strains.

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