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Originally published as Genetics Published Articles Ahead of Print on August 24, 2007.
Genetics, Vol. 177, 615-629, September 2007, Copyright © 2007
doi:10.1534/genetics.107.076216
The DrosDel Deletion Collection: A Drosophila Genomewide Chromosomal Deficiency Resource
Edward Ryder*,
Michael Ashburner*,
Rosa Bautista-Llacer*,
Jenny Drummond*,
Jane Webster*,
Glynnis Johnson*,
Terri Morley*,
Yuk Sang Chan*,
Fiona Blows*,
Darin Coulson*,
Gunter Reuter
,
Heiko Baisch,
Christian Apelt,
Andreas Kauk,
Thomas Rudolph,
Maria Kube,
Melanie Klimm,
Claudia Nickel,
Janos Szidonya
,
Peter Maróy,
Margit Pal
,
Åsa Rasmuson-Lestander**,
Karin Ekström**,
Hugo Stocker,
Christoph Hugentobler,
Ernst Hafen,
David Gubb,
Gert Pflugfelder,
Christian Dorner,
Bernard Mechler***,
Heide Schenkel***,
Joachim Marhold***,
Florenci Serras,
Montserrat Corominas,
Adrià Punset,
John Roote* and
Steven Russell*,1
* Department of Genetics, University of Cambridge, Cambridge CB2 3EH, United Kingdom, Institute of Biology/Genetics, Martin Luther University, D-06120 Halle, Germany, Department of Genetics and Molecular Biology, University of Szeged, 6726 Szeged, Hungary, Institute of Biochemistry, Biological Research Center, Szeged H-6726, Hungary, ** Department of Molecular Biology, Umeå University, S-901 87 Umeå, Sweden, Zoologisches Institut der Universitat Zurich, 8057 Zurich, Switzerland, CIC Biogune, Parque Technologico de Bizkaia, Derio 48160, Spain, Institut für Genetik, Universität Mainz, 55128 Mainz, Germany, *** Department of Developmental Genetics, Deutsches Krebsforschungszentrum, D-69120 Heidelberg, Germany and Departament de Genetica, Facultat de Biologia, Universitat de Barcelona, 08028 Barcelona, Spain
1 Corresponding author: Department of Genetics, University of Cambridge, Downing St., Cambridge CB2 3EH, United Kingdom.
E-mail: s.russell{at}gen.cam.ac.uk
We describe a second-generation deficiency kit for Drosophila melanogaster composed of molecularly mapped deletions on an isogenic background, covering 
77% of the Release 5.1 genome. Using a previously reported collection of FRT-bearing P-element insertions, we have generated 655 new deletions and verified a set of 209 deletion-bearing fly stocks. In addition to deletions, we demonstrate how the P elements may also be used to generate a set of custom inversions and duplications, particularly useful for balancing difficult regions of the genome carrying haplo-insufficient loci. We describe a simple computational resource that facilitates selection of appropriate elements for generating custom deletions. Finally, we provide a computational resource that facilitates selection of other mapped FRT-bearing elements that, when combined with the DrosDel collection, can theoretically generate over half a million precisely mapped deletions.
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Genetics 2007 177: NP.
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