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Originally published as Genetics Published Articles Ahead of Print on July 1, 2007.
Genetics, Vol. 177, 17-30, September 2007, Copyright © 2007
doi:10.1534/genetics.107.074708
Topoisomerase I-Dependent Viability Loss in Saccharomyces cerevisiae Mutants Defective in Both SUMO Conjugation and DNA Repair
Xiaole L. Chen, Hannah R. Silver, Ling Xiong, Irina Belichenko, Caroline Adegite and Erica S. Johnson1
Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107
1 Corresponding author: Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 S. 10th St., BLSB 231, Philadelphia, PA 19107.
E-mail: erica.johnson{at}jefferson.edu
Siz1 and Siz2/Nfi1 are the two Siz/PIAS SUMO E3 ligases in Saccharomyces cerevisiae. Here we show that siz1
siz2 mutants fail to grow in the absence of the homologous recombination pathway or the Fen1 ortholog RAD27. Remarkably, the growth defects of mutants such as siz1 siz2 rad52 are suppressed by mutations in TOP1, suggesting that these growth defects are caused by topoisomerase I activity. Other mutants that affect SUMO conjugation, including a ulp1 mutant and the nuclear pore mutants nup60 and nup133, show similar top1-suppressible synthetic defects with DNA repair mutants, suggesting that these phenotypes also result from reduced SUMO conjugation. siz1 siz2 mutants also display TOP1-independent genome instability phenotypes, including increased mitotic recombination and elongated telomeres. We also show that SUMO conjugation, TOP1, and RAD27 have overlapping roles in telomere maintenance. Top1 is sumoylated, but Top1 does not appear to be the SUMO substrate involved in the synthetic growth defects. However, sumoylation of certain substrates, including Top1 itself and Tri1 (YMR233W), is enhanced in the absence of Top1 activity. Sumoylation is also required for growth of top1 cells. These results suggest that the SUMO pathway has a complex effect on genome stability that involves several mechanistically distinct processes.
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