Originally published as Genetics Published Articles Ahead of Print on July 1, 2007.

Genetics, Vol. 176, 2651-2655, August 2007, Copyright © 2007
doi:10.1534/genetics.107.074369

Temporal Control of Cell-Specific Transgene Expression in Caenorhabditis elegans

Taulant Bacaj and Shai Shaham¹

Laboratory of Developmental Genetics, The Rockefeller University, New York, New York 10021

¹ Corresponding author: Laboratory of Developmental Genetics, The Rockefeller University, 1230 York Ave., New York, NY 10021.
E-mail: shaham{at}rockefeller.edu

Cell-specific promoters allow only spatial control of transgene expression in Caenorhabditis elegans. We describe a method, using cell-specific rescue of heat-shock factor-1 (hsf-1) mutants, that allows spatial and temporal regulation of transgene expression. We demonstrate the utility of this method for timed reporter gene expression and for temporal studies of gene function.

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