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Originally published as Genetics Published Articles Ahead of Print on July 1, 2007.
Genetics, Vol. 176, 2279-2291, August 2007, Copyright © 2007
doi:10.1534/genetics.107.074088
A Clonal Genetic Screen for Mutants Causing Defects in Larval Tracheal Morphogenesis in Drosophila
Magdalena M. Baer1, Andreas Bilstein1 and Maria Leptin2
Institute of Genetics, University of Cologne, D-50674 Cologne, Germany
2 Corresponding author: Institute of Genetics, University of Cologne, Zülpicher Str. 47, D-50674 Cologne, Germany.
E-mail: mleptin{at}uni-koeln.de
The initial establishment of the tracheal network in the Drosophila embryo is beginning to be understood in great detail, both in its genetic control cascades and in its cell biological events. By contrast, the vast expansion of the system during larval growth, with its extensive ramification of preexisting tracheal branches, has been analyzed less well. The mutant phenotypes of many genes involved in this process are probably not easy to reveal, as these genes may be required for other functions at earlier developmental stages. We therefore conducted a screen for defects in individual clonal homozygous mutant cells in the tracheal network of heterozygous larvae using the mosaic analysis with a repressible cell marker (MARCM) system to generate marked, recombinant mitotic clones. We describe the identification of a set of mutants with distinct phenotypic effects. In particular we found a range of defects in terminal cells, including failure in lumen formation and reduced or extensive branching. Other mutations affect cell growth, cell shape, and cell migration.
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Genetics 2007 176: NP.
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