help button home button Genetics Proc NAS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Originally published as Genetics Published Articles Ahead of Print on June 11, 2007.

Genetics, Vol. 176, 2055-2067, August 2007, Copyright © 2007
doi:10.1534/genetics.107.074286

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Data Supplement
Right arrow All Versions of this Article:
genetics.107.074286v1
176/4/2055    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stupar, R. M.
Right arrow Articles by Jiang, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stupar, R. M.
Right arrow Articles by Jiang, J.

Phenotypic and Transcriptomic Changes Associated With Potato Autopolyploidization

Robert M. Stupar*,1, Pudota B. Bhaskar*, Brian S. Yandell*,{dagger}, Willem A. Rensink{ddagger}, Amy L. Hart{ddagger}, Shu Ouyang{ddagger}, Richard E. Veilleux§, James S. Busse**, Robert J. Erhardt{dagger}, C. Robin Buell{ddagger} and Jiming Jiang*,2

* Department of Horticulture, University of Wisconsin, Madison, Wisconsin 53706 and {dagger} Department of Statistics, University of Wisconsin, Madison, Wisconsin 53706 and {ddagger} The Institute for Genomic Research, Rockville, Maryland 20850 and § Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061 and ** USDA–ARS, Vegetable Crops Research Unit, Madison, Wisconsin 53706

2 Corresponding author: Department of Horticulture, University of Wisconsin, Madison, WI 53706.
E-mail: jjiang1{at}wisc.edu

Polyploidy is remarkably common in the plant kingdom and polyploidization is a major driving force for plant genome evolution. Polyploids may contain genomes from different parental species (allopolyploidy) or include multiple sets of the same genome (autopolyploidy). Genetic and epigenetic changes associated with allopolyploidization have been a major research subject in recent years. However, we know little about the genetic impact imposed by autopolyploidization. We developed a synthetic autopolyploid series in potato (Solanum phureja) that includes one monoploid (1x) clone, two diploid (2x) clones, and one tetraploid (4x) clone. Cell size and organ thickness were positively correlated with the ploidy level. However, the 2x plants were generally the most vigorous and the 1x plants exhibited less vigor compared to the 2x and 4x individuals. We analyzed the transcriptomic variation associated with this autopolyploid series using a potato cDNA microarray containing ~9000 genes. Statistically significant expression changes were observed among the ploidies for ~10% of the genes in both leaflet and root tip tissues. However, most changes were associated with the monoploid and were within the twofold level. Thus, alteration of ploidy caused subtle expression changes of a substantial percentage of genes in the potato genome. We demonstrated that there are few genes, if any, whose expression is linearly correlated with the ploidy and can be dramatically changed because of ploidy alteration.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Hovav, J. A. Udall, B. Chaudhary, R. Rapp, L. Flagel, and J. F. Wendel
Partitioned expression of duplicated genes during development and evolution of a single cell in a polyploid plant
PNAS, April 22, 2008; 105(16): 6191 - 6195.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2007 by the Genetics Society of America.