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Originally published as Genetics Published Articles Ahead of Print on May 4, 2007.
Genetics, Vol. 176, 1907-1921, July 2007, Copyright © 2007
doi:10.1534/genetics.107.072157
Centimorgan-Range One-Step Mapping of Fertility Traits Using Interspecific Recombinant Congenic Mice
David L'Hôte*,
,
Catherine Serres
,
Paul Laissue*,
Ahmad Oulmouden,
Claire Rogel-Gaillard
,
Xavier Montagutelli** and
Daniel Vaiman*,,1
* Equipe 21, Génomique et Epigénétique des Pathologies Placentaires, Unité INSERM 567/UMR CNRS 8104-Université Paris V IFR Alfred Jost, Faculté de Médecine, 75014 Paris, France, Université Paris Descartes, Faculté de Médecine de Cochin, Biologie de la Reproduction, 75014 Paris, France, Unité de Genetique Moleculaire Animale, UMR 1061-INRA/Universite de Limoges, 87000 Limoges, France, ** Unité de Genetique des Mammiferes, Institut Pasteur, 75724 Paris Cedex 15, France and Animal Genetics Department, INRA, France
1 Corresponding author: Génomique et Epigénétique des Pathologies Placentaires, Unité INSERM 567/UMR CNRS 8104-Université Paris V IFR Alfred Jost, Faculté de Médecine, 24 rue du faubourg Saint Jacques, 75014 Paris, France.
E-mail: vaiman{at}cochin.inserm.fr
In mammals, male fertility is a quantitative feature determined by numerous genes. Until now, several wide chromosomal regions involved in fertility have been defined by genetic mapping approaches; unfortunately, the underlying genes are very difficult to identify. Here, 53 interspecific recombinant congenic mouse strains (IRCSs) bearing 1–2% SEG/Pas (Mus spretus) genomic fragments disseminated in a C57Bl/6J (Mus domesticus) background were used to systematically analyze male fertility parameters. One of the most prominent advantages of this model is the possibility of analyzing stable phenotypes in living animals. Here, we demonstrate the possibility in one-step fine mapping for several fertility traits. Focusing on strains harboring a unique spretus fragment, we could unambiguously localize two testis and one prostate weight-regulating QTL (Ltw1, Ltw2, and Lpw1), four QTL controlling the sperm nucleus shape (Sh1, Sh2, Sh3, and Sh4), and one QTL influencing sperm survival (Dss1). In several cases, the spretus DNA fragment was small enough to propose sound candidates. For instance, Spata1, Capza, and Tuba7 are very strong candidates for influencing the shape of the sperm head. Identifying new genes implied in mammalian fertility pathways is a necessary prerequisite for clarifying their molecular grounds and for proposing diagnostic tools for masculine infertilities.
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