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Originally published as Genetics Published Articles Ahead of Print on April 3, 2007.
Genetics, Vol. 176, 841-852, June 2007, Copyright © 2007
doi:10.1534/genetics.106.069732
Inorganic Phosphate Deprivation Causes tRNA Nuclear Accumulation via Retrograde Transport in Saccharomyces cerevisiae
Rebecca L. Hurto*,
Amy Hin Yan Tong
,
Charles Boone and
Anita K. Hopper*,1
* Department of Molecular Genetics, Ohio State University, Columbus, Ohio 43210 and Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario M5G 1L6, Canada
1 Corresponding author: Department of Molecular Genetics, Ohio State University, 484 W. 12th Ave., Columbus, OH 43210.
E-mail: hopper.64{at}osu.edu
Nuclear export of tRNA is an essential eukaryotic function, yet the one known yeast tRNA nuclear exporter, Los1, is nonessential. Moreover recent studies have shown that tRNAs can move retrograde from the cytosol to the nucleus by an undefined process. Therefore, additional gene products involved in tRNA nucleus–cytosol dynamics have yet to be identified. Synthetic genetic array (SGA) analysis was employed to identify proteins involved in Los1-independent tRNA transport and in regulating tRNA nucleus–cytosol distribution. These studies uncovered synthetic interactions between los1
and pho88 involved in inorganic phopshate uptake. Further analysis revealed that inorganic phosphate deprivation causes transient, temperature-dependent nuclear accumulation of mature cytoplasmic tRNA within nuclei via a Mtr10- and retrograde-dependent pathway, providing a novel connection between tRNA subcellular dynamics and phosphate availability.
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Genetics 2007 176: NP.
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