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Originally published as Genetics Published Articles Ahead of Print on December 18, 2006.
Genetics, Vol. 176, 711-714, May 2007, Copyright © 2007
doi:10.1534/genetics.106.063610
Estimation of Population Heterozygosity and Library Construction-Induced Mutation Rate From Expressed Sequence Tag Collections
A. D. Long*,1,
P. Beldade
and
S. J. Macdonald*,
* Ecology and Evolutionary Biology, University of California, Irvine, California 92697, Institute of Biology, University of Leiden, 63 2311 GP Leiden, The Netherlands and Department of Ecology and Evolutionary Biology and Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas 66045
1 Corresponding author: Ecology and Evolutionary Biology, 321 Steinhaus Hall, University of California, Irvine, CA 92697.
E-mail: tdlong{at}uci.edu
Unigene alignments obtained from cDNA libraries made using multiple individuals are not currently used to estimate population heterozygosity, as they are known to harbor mutations created during library construction. We describe an estimator of population heterozygosity that utilizes only SNPs unlikely to be library construction artifacts.