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Originally published as Genetics Published Articles Ahead of Print on December 28, 2006.
Genetics, Vol. 176, 697-702, May 2007, Copyright © 2007
doi:10.1534/genetics.106.069666
An Unusual Pattern of Spontaneous Mutations Recovered in the Halophilic Archaeon Haloferax volcanii
Reena R. Mackwan*,
Geraldine T. Carver
,
John W. Drake and
Dennis W. Grogan*,1
* Department of Biological Sciences, University of Cincinnati, Cincinnati, Ohio 45221-0006 and Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709-2233
1 Corresponding author: Department of Biological Sciences, University of Cincinnati, 614 Rieveschl Hall, Cincinnati, OH 45221-0006.
E-mail: grogandw{at}email.uc.edu
Spontaneous mutations in the orotate:phosphoribosyl transferase (pyrE2) gene of the halophilic archaeon Haloferax volcanii were selected by 5-fluoroorotic acid plus uracil at a rate of 
2 x 10–8/cell division in fluctuation and null-fraction tests but 6 x 10–8/cell division in mutation-accumulation tests. The corresponding genomic mutation rates were substantially lower than those observed for other mesophilic microbial DNA genomes on the basis of similar target genes. The mutational spectrum was dominated by indels adding or deleting multiples of 3 bp. Properties of the organism contributing to this unusual mutational pattern may include phenotypic lag caused by a high chromosomal copy number and efficient promotion of strand misalignments by short direct repeats.
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