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Originally published as Genetics Published Articles Ahead of Print on March 4, 2007.
Genetics, Vol. 176, 43-52, May 2007, Copyright © 2007
doi:10.1534/genetics.106.069161
Neurospora Spore Killers Sk-2 and Sk-3 Suppress Meiotic Silencing by Unpaired DNA
Namboori B. Raju*,1,
Robert L. Metzenberg
and
Patrick K. T. Shiu
* Department of Biological Sciences, Stanford University, Stanford, California 94305, Department of Biology, California State University, Northridge, California 91330 and Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211
1 Corresponding author: Department of Biological Sciences, Stanford University, Stanford, CA 94305-5020.
E-mail: nbraju{at}stanford.edu
In Neurospora crassa, pairing of homologous DNA segments is monitored during meiotic prophase I. Any genes not paired with a homolog, as well as any paired homologs of that gene, are silenced during the sexual phase by a mechanism known as meiotic silencing by unpaired DNA (MSUD). Two genes required for MSUD have been described previously: sad-1 (suppressor of ascus dominance), encoding an RNA-directed RNA polymerase, and sad-2, encoding a protein that controls the perinuclear localization of SAD-1. Inactivation of either sad-1 or sad-2 suppresses MSUD. We have now shown that MSUD is also suppressed by either of two Spore killer strains, Sk-2 and Sk-3. These were both known to contain a haplotype segment that behaves as a meiotic drive element in heterozygous crosses of killer x sensitive. Progeny ascospores not carrying the killer element fail to mature and are inviable. Crosses homozygous for either of the killer haplotypes suppress MSUD even though ascospores are not killed. The killer activity maps to the same 30-unit-long region within which recombination is suppressed in killer x sensitive crosses. We suggest that the region contains a suppressor of MSUD.
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Genetics 2007 176: NP.
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