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Originally published as Genetics Published Articles Ahead of Print on December 18, 2006.
Genetics, Vol. 175, 1707-1718, April 2007, Copyright © 2007
doi:10.1534/genetics.106.064212
Misexpression Screen in Drosophila melanogaster Aiming to Reveal Novel Factors Involved in Formation of Body Parts
Nicole C. Grieder*,1,
Ilias Charlafti*,
Urs Kloter*,
Herbert Jäckle
,
Ulrich Schäfer and
Walter J. Gehring*
* Biozentrum der Universität Basel, Abteilung Zellbiologie, CH-4056 Basel, Switzerland and Max-Planck Institut für Biophysikalische Chemie, Abteilung Molekulare Entwicklungsbiologie, D-37077 Göttingen, Germany
1 Corresponding author: Biozentrum der Universität Basel, Abteilung Zellbiologie, Klingelbergstrasse 50-70, CH-4056 Basel, Switzerland.
E-mail: nicole.grieder{at}unibas.ch
To identify novel factors that lead a fly imaginal disc to adopt its developmental fate, we carried out a modular dominant misexpression screen in imaginal discs. We have identified two factors that appear to change the fate of the respective body structure and appear to lead to the transformation of a body part. In one mutant line, notum tissue, normally derived from wing imaginal tissue, formed close to the site of the sternopleural bristles, which are leg disc derivatives. In the other line, the arista is transformed into a tubular structure, resembling an abnormal leg. We found that ectopic expression of abrupt was responsible for this potential transformation of the arista.